Kovács K A, Oszter A, Göcze P M, Környei J L, Szabó I
Department of Obstetrics and Gynecology, Pécs University Medical School, Edesanyák u.13/15, Pécs, H-7624, Hungary.
Mol Hum Reprod. 2001 Nov;7(11):1085-91. doi: 10.1093/molehr/7.11.1085.
The aim of these experiments was to investigate the expression of cyclin D1 and of oestradiol receptors as well as the level of [(3)H]oestradiol binding in leiomyoma and adjacent myometrium from human uteri at different menstrual phases and at an early stage of menopause. [(3)H]oestradiol binding was determined by saturation analysis, while the oestradiol receptor (ER) alpha and beta and cyclin D1 levels were determined by Western blot analysis of 16 samples of human leiomyomas and corresponding myometria at different hormonal stages. In leiomyomas during all phases of the menstrual cycle, ERalpha expression, high affinity oestradiol binding and cyclin D1 expression were all elevated in comparison with adjacent myometrium. ERbeta expression and low affinity oestradiol binding were enhanced in leiomyomas only during the proliferative phase. During menopause, ERbeta expression and low affinity binding were enhanced in leiomyomas, while the ERalpha expression was not significantly enhanced and cyclin D1 levels were similar to that in myometrium. Only the oestradiol binding exhibited any menstrual cycle-related changes. Our data suggest the involvement of cyclin D1 in the growth of leiomyomas during the menstrual cycle. In menopause, there appears to be a switch from ERalpha to ERbeta expression in leiomyomas, and the induction of cyclin D1 is decreased. The regression of tumour may ensue from these changes at menopause.
这些实验的目的是研究细胞周期蛋白D1和雌二醇受体的表达，以及在不同月经周期阶段和绝经早期的人子宫平滑肌瘤及相邻子宫肌层中[³H]雌二醇结合水平。通过饱和分析测定[³H]雌二醇结合，而通过对16份处于不同激素阶段的人平滑肌瘤及相应子宫肌层样本进行蛋白质免疫印迹分析来测定雌二醇受体（ER）α和β以及细胞周期蛋白D1水平。在月经周期的所有阶段，与相邻子宫肌层相比，平滑肌瘤中的ERα表达、高亲和力雌二醇结合和细胞周期蛋白D1表达均升高。仅在增殖期，平滑肌瘤中的ERβ表达和低亲和力雌二醇结合增强。在绝经期间，平滑肌瘤中的ERβ表达和低亲和力结合增强，而ERα表达未显著增强且细胞周期蛋白D1水平与子宫肌层相似。只有雌二醇结合呈现出与月经周期相关的变化。我们的数据表明细胞周期蛋白D1参与月经周期中平滑肌瘤的生长。在绝经时，平滑肌瘤中似乎存在从ERα表达向ERβ表达的转变，并且细胞周期蛋白D1的诱导减少。绝经时的这些变化可能导致肿瘤消退。
