Környei J, Csermely T, Székely J A, Vértes M
Exp Clin Endocrinol. 1986 Aug;87(3):256-64. doi: 10.1055/s-0029-1210554.
Two types of oestradiol binding sites were identified in human myometrium and myoma by saturation analysis. Nuclear type I receptors bind Oe with high affinity (KD = 1.4 nM) and low capacity (0.1-2.0 pmole/mg DNA) in a competitive fashion (Hill coeff. = 1.0), while nuclear type II sites bind the hormone with reduced affinity (KD: approximately 20 nM in myometrium and KD: approximately 40 nM in myoma), but high capacity (1-15 pmole/mg DNA) by positive cooperation (Hill coeff.: 4-5 in myometrium and 2-3 in myoma). Binding properties of type II sites in myoma are similar to those found in endometrium in previous studies of our laboratory, rather than those found in normal myometrium. The concentration of both nuclear Oe binding sites varied with the menstrual cycle. In myometrium, maximal concentration of nuclear type I and type II sites occurred between 10-14 days of cycle, when blood Oe level is the highest. In myoma the concentration of type I receptors was highest during the late follicular phase, but type II sites were uniformly high during the first 14 days of the cycle. In the luteal phase, receptor concentrations were low and apparently unaltered. It is possible that these changes in Oe binding capability of leiomyoma play a role in the pathomechanism.
通过饱和分析在人子宫肌层和子宫肌瘤中鉴定出两种雌二醇结合位点。核I型受体以竞争性方式（希尔系数=1.0）高亲和力（KD = 1.4 nM）且低容量（0.1 - 2.0 pmole/mg DNA）结合雌二醇，而核II型位点以正协同作用（子宫肌层中希尔系数为4 - 5，肌瘤中为2 - 3）低亲和力（子宫肌层中KD约为20 nM，肌瘤中KD约为40 nM）但高容量（1 - 15 pmole/mg DNA）结合该激素。肌瘤中II型位点的结合特性与我们实验室先前研究中在子宫内膜中发现的相似，而非正常子宫肌层中的特性。两种核雌二醇结合位点的浓度随月经周期而变化。在子宫肌层中，核I型和II型位点的最大浓度出现在月经周期的10 - 14天之间，此时血液中雌二醇水平最高。在肌瘤中，I型受体浓度在卵泡晚期最高，但II型位点在周期的前14天一直保持较高水平。在黄体期，受体浓度较低且显然未改变。肌瘤中雌二醇结合能力的这些变化可能在发病机制中起作用。
