Ribas de Pouplana L, Brown J R, Schimmel P
Skaggs Institute for Chemical Biology, Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
J Mol Evol. 2001 Oct-Nov;53(4-5):261-8. doi: 10.1007/s002390010216.
The available three-dimensional information for class II aminoacyl-tRNA synthetases has been used to generate sequence alignments that strictly adhere to the structural equivalencies between members of subclass IIa of these enzymes. The resulting alignments were used to study their phylogenetic relationships. In particular, the entire set of available sequences of prolyl-tRNA synthetases was analyzed in this way. In contrast to recent reports, we conclude that the evolutionary pattern of prolyl-tRNA synthetases does not obviously conform to the canonical phylogenetic distribution. The pattern found for these enzymes may be related to their biochemical characteristics. Our results indicate a potential relationship between the evolutionary pattern of prolyl-tRNA synthetases and the emergence of two enzymatically distinct forms of these proteins.
II类氨酰-tRNA合成酶的现有三维信息已用于生成序列比对，这些比对严格遵循这些酶IIa亚类成员之间的结构等效性。所得比对用于研究它们的系统发育关系。特别是，以这种方式分析了脯氨酰-tRNA合成酶的整套可用序列。与最近的报道相反，我们得出结论，脯氨酰-tRNA合成酶的进化模式并不明显符合典型的系统发育分布。这些酶的模式可能与其生化特性有关。我们的结果表明脯氨酰-tRNA合成酶的进化模式与这些蛋白质的两种酶学上不同形式的出现之间存在潜在关系。
