8-异戊烯基柚皮素，啤酒花和啤酒中的植物雌激素，上调人乳腺癌细胞中E-钙黏蛋白/连环蛋白复合物的功能。

8-Prenylnaringenin, the phytoestrogen in hops and beer, upregulates the function of the E-cadherin/catenin complex in human mammary carcinoma cells.

作者信息

Rong H, Boterberg T, Maubach J, Stove C, Depypere H, Van Slambrouck S, Serreyn R, De Keukeleire D, Mareel M, Bracke M

机构信息

Laboratory of Pharmacognosy and Phytochemistry, Faculty of Pharmaceutical Sciences, University Gent, Belgium.

出版信息

Eur J Cell Biol. 2001 Sep;80(9):580-5. doi: 10.1078/0171-9335-00190.

DOI:10.1078/0171-9335-00190

PMID:11675933

Abstract

The E-cadherin/catenin complex is a powerful invasion suppressor in epithelial cells. It is expressed in the human MCF-7 breast cancer cell line family, but functionally defective in the invasive MCF-7/6 variant. Previous experiments have shown that IGF-I, tamoxifen, retinoic acid and tangeretin are able to upregulate the function of this complex in MCF-7/6 cells. We investigated the effect of 8-prenylnaringenin (8-PN), the phytoestrogen present in hops and beer, on aggregation, growth and invasion in MCF-7/6 cells. 8-PN was found to stimulate E-cadherin-dependent aggregation and growth of MCF-7/6 cells in suspension. These effects could be inhibited by the pure anti-estrogen ICI 182,780. 8-PN did not affect invasion of MCF-7/6 cells in the chick heart assay in vitro. In all these aspects 8-PN mimics the effects of 17beta-estradiol on MCF-7/6 cells.

摘要

E-钙黏蛋白/连环蛋白复合体是上皮细胞中一种强大的侵袭抑制因子。它在人MCF-7乳腺癌细胞系家族中表达，但在侵袭性的MCF-7/6变体中功能存在缺陷。先前的实验表明，胰岛素样生长因子-I（IGF-I）、他莫昔芬、视黄酸和橘红素能够上调MCF-7/6细胞中该复合体的功能。我们研究了啤酒花和啤酒中含有的植物雌激素8-异戊烯基柚皮素（8-PN）对MCF-7/6细胞聚集、生长和侵袭的影响。发现8-PN能刺激MCF-7/6细胞在悬浮状态下依赖E-钙黏蛋白的聚集和生长。这些作用可被纯抗雌激素ICI 182,780抑制。在体外鸡胚心脏试验中，8-PN不影响MCF-7/6细胞的侵袭。在所有这些方面，8-PN模拟了17β-雌二醇对MCF-7/6细胞的作用。