Kurz C, Tempfer C B, Boecskoer S, Unfried G, Nagele F, Hefler L A
Department of Endocrinology and Reproductive Medicine, University of Vienna School of Medicine, Vienna, Austria.
J Soc Gynecol Investig. 2001 Sep-Oct;8(5):295-8. doi: 10.1016/s1071-5576(01)00123-x.
Progesterone inhibits lymphocyte cytotoxicity, natural killer cell degranulation, and release of proinflammatory cytokines and has been shown to protect against spontaneous miscarriage. We investigated the association between idiopathic recurrent miscarriage (IRM) and the PROGINS 306 base pair insertion polymorphism in intron G of the progesterone receptor gene, which is known to segregate with progesterone-dependent neoplasms.
In a case-control study we investigated 125 women with a history of three or more consecutive pregnancy losses before 20 weeks' gestation and 79 healthy controls with at least two live births and no history of pregnancy loss. Peripheral venous puncture, DNA extraction, and polymerase chain reaction were used to genotype women for the presence of the PROGINS polymorphism.
Allele frequencies among women with IRM and controls were 85.2% and 89.2%, respectively, for allele T1 (wild type) and 14.8% and 10.8%, respectively, for allele T2 (mutant). No association between allele T2 and the occurrence of IRM was found (P =.3; odds ratio [OR] 0.69; confidence interval [CI] 0.34, 1.40). Genotype frequencies were not significantly different between the study group (T1/T1 73.6%, T1/T2 23.2%, T2/T2 3.2%) and the control group (T1/T1 79.7%, T1/T2 19%, T2/T2 1.3%) (P =.4). Between women with primary and secondary IRM, there were no statistically significant differences with respect to allele frequencies (82% versus 87%, P =.4 for allele T1 and 12% versus 13%, P =.6 for allele T2).
We found that the PROGINS polymorphism in the progesterone receptor gene was not associated with IRM in white women.
孕酮可抑制淋巴细胞的细胞毒性、自然杀伤细胞的脱颗粒作用以及促炎细胞因子的释放，并且已被证明可预防自然流产。我们研究了特发性复发性流产（IRM）与孕酮受体基因内含子G中PROGINS 306碱基对插入多态性之间的关联，已知该多态性与孕酮依赖性肿瘤相关。
在一项病例对照研究中，我们调查了125名在妊娠20周前有三次或更多次连续妊娠丢失史的女性，以及79名至少有两次活产且无妊娠丢失史的健康对照者。通过外周静脉穿刺、DNA提取和聚合酶链反应对女性进行基因分型，以确定是否存在PROGINS多态性。
IRM女性和对照者中，等位基因T1（野生型）的频率分别为85.2%和89.2%，等位基因T2（突变型）的频率分别为14.8%和10.8%。未发现等位基因T2与IRM的发生之间存在关联（P = 0.3；优势比[OR] 0.69；置信区间[CI] 0.34，1.40）。研究组（T1/T1 73.6%，T1/T2 23.2%，T2/T2 3.2%）和对照组（T1/T1 79.7%，T1/T2 19%，T2/T2 1.3%）之间的基因型频率无显著差异（P = 0.4）。在原发性和继发性IRM女性之间，等位基因频率无统计学显著差异（等位基因T1为82%对87%，P = 0.4；等位基因T2为12%对13%，P = 0.6）。
我们发现孕酮受体基因中的PROGINS多态性与白人女性的IRM无关。
