Oliveira Tenilson Amaral, da Cunha Danielle Renzoni, Policastro Adriana, Traina Évelyn, Gomes Mariano Tamura, Cordioli Eduardo
Hospital Leonor Mendes de Barros, São Paulo, SP, Brasil.
Rev Bras Ginecol Obstet. 2011 Jun;33(6):271-5.
to investigate the association between gene polymorphism of the progesterone receptor (PROGINS) and the risk of premature birth.
In this case-control study, 57 women with previous premature delivery (Case Group) and 57 patients with delivery at term in the current pregnancy and no history of preterm delivery (Control Group) were selected. A 10 mL amount of peripheral blood was collected by venipuncture and genomic DNA was extracted followed by the polymerase chain reaction (PCR) under specific conditions for this polymorphism and 2% agarose gel electrophoresis. The bands were visualized with an ultraviolet light transilluminator. Genotype and allele PROGINS frequencies were compared between the two groups by the χ2 test, with the level of significance set at value p < 0.05. The Odds Ratio (OR) was also used, with 95% confidence intervals.
PROGINS genotypic frequencies were 75.4% T1/T1, 22.8% T1/T2 and 1.8% T2/T2 in the Group with Preterm Delivery and 80.7% T1/T1, 19.3% T1/T2 and 0% T2/T2 in the term Delivery Group. There were no differences between groups when genotype and allele frequencies were analyzed: p = 0.4 (OR = 0.7) and p = 0.4 (OR = 0.7).
the present study suggests that the presence of PROGINS polymorphism in our population does not constitute a risk factor for premature birth.
探讨孕激素受体（PROGINS）基因多态性与早产风险之间的关联。
在这项病例对照研究中，选取了57例有早产史的女性（病例组）和57例本次妊娠足月分娩且无早产史的患者（对照组）。通过静脉穿刺采集10 mL外周血，提取基因组DNA，然后在特定条件下进行针对该多态性的聚合酶链反应（PCR）及2%琼脂糖凝胶电泳。用紫外光透射仪观察条带。采用χ2检验比较两组之间PROGINS基因型和等位基因频率，显著性水平设定为p < 0.05。还使用了比值比（OR）及95%置信区间。
早产组中PROGINS基因型频率分别为T1/T1 75.4%、T1/T2 22.8%和T2/T2 1.8%，足月分娩组中分别为T1/T1 80.7%、T1/T2 19.3%和T2/T2 0%。分析基因型和等位基因频率时，两组之间无差异：p = 0.4（OR = 0.7）和p = 0.4（OR = 0.7）。
本研究表明，在我们的人群中PROGINS多态性的存在不构成早产的危险因素。
