Witz C A, Montoya-Rodriguez I A, Cho S, Centonze V E, Bonewald L F, Schenken R S
Department of Obstetrics and Gynecology, The University of Texas Health Science Center at San Antonio, San Antonio, Texas 78229-3900, USA.
J Soc Gynecol Investig. 2001 Sep-Oct;8(5):299-304. doi: 10.1016/s1071-5576(01)00122-8.
To localize the extracellular matrix proteins collagen I, collagen IV, fibronectin, and laminin in the peritoneal membrane.
Peritoneal biopsies (n = 13) from the anterior abdominal wall and the uterine serosa (n = 3) were incubated with antibodies to collagen IV, laminin, collagen I, and fibronectin. Specimens were examined using light and confocal laser scanning microscopy.
All of the extracellular matrix (ECM) proteins were present immediately under the mesothelium. Collagen (Col) IV and laminin (LM) were seen in the smooth muscle of microvascular structures, in the subendothelial basement membrane, and were present in a fascicular pattern in the peritoneal stroma. Collagen I was distributed diffusely in the peritoneal stroma. Fibronectin was also present in the subendothelial basement membrane.
The resolution of the confocal microscope allowed for localization of extracellular matrix proteins in relation to the mesothelium. The presence of collagen IV, laminin, collagen I, and fibronectin under the mesothelium suggests that cells invading the peritoneum must have the ability to degrade and remodel this matrix.
在腹膜中定位细胞外基质蛋白I型胶原、IV型胶原、纤连蛋白和层粘连蛋白。
将取自前腹壁的腹膜活检标本（n = 13）和子宫浆膜的腹膜活检标本（n = 3）与抗IV型胶原、层粘连蛋白、I型胶原和纤连蛋白的抗体一起孵育。使用光学显微镜和共聚焦激光扫描显微镜检查标本。
所有细胞外基质（ECM）蛋白均位于间皮下方。IV型胶原（Col）和层粘连蛋白（LM）可见于微血管结构的平滑肌、内皮下基底膜，并以束状模式存在于腹膜基质中。I型胶原弥漫分布于腹膜基质中。纤连蛋白也存在于内皮下基底膜中。
共聚焦显微镜的分辨率使得能够确定细胞外基质蛋白相对于间皮的定位。间皮下存在IV型胶原、层粘连蛋白、I型胶原和纤连蛋白表明，侵入腹膜的细胞必须具有降解和重塑这种基质的能力。
