Symington J, Schlesinger M J
J Virol. 1975 Apr;15(4):1037-41. doi: 10.1128/JVI.15.4.1037-1041.1975.
A variant of Sindbis virus has been isolated by growing a stock of virus, previously passaged on chicken embryo cells, in mouse plasmacytoma (MOPC 315) cells in suspension culture. An indirect immunofluorescence test and infective center assay showed that only a small fraction of cells could be infected by the stock wild-type virus, but that the population of virus accumulating after a few passages on the mouse cells had host-range properties distinct from the stock virus. The mouse-passaged virus retained its virulence for the original host and back-passaging of this virus on chicken cells did not change its newly acquired properties. Thus, this variant appears to be a genetically distinct form of Sindbis that adsorbs to and grows much better than the stock virus on several types of mouse cells including cultures of mouse macrophages.
通过在悬浮培养的小鼠浆细胞瘤（MOPC 315）细胞中培养一批先前在鸡胚细胞上传代的病毒株，分离出了辛德毕斯病毒的一个变种。间接免疫荧光试验和感染中心测定表明，只有一小部分细胞能被该原始野生型病毒株感染，但在小鼠细胞上传代几次后积累的病毒群体具有与原始病毒株不同的宿主范围特性。在小鼠中传代的病毒对原始宿主仍保持其毒力，并且将该病毒再接种到鸡细胞上并没有改变其新获得的特性。因此，这种变种似乎是一种基因上不同的辛德毕斯病毒形式，它在包括小鼠巨噬细胞培养物在内的几种类型的小鼠细胞上比原始病毒株吸附得更好且生长得更好。
