Igarashi A, Stollar V
J Virol. 1976 Aug;19(2):398-408. doi: 10.1128/JVI.19.2.398-408.1976.
Whereas defective interfering particles of Sindbis virus are readily produced in BHK-21 cells or chicken embryo fibroblasts by the techniques of serial undiluted passage, similar methods failed to generate such particles in Aedes albopictus cell cultures. In addition, Sindbis virus stocks produced in BHK-21 cells or chicken embryo fibroblasts and which contained defective interfering particles, when tested in A. albopictus cells, failed (i) to interfere with the replication of standard Sindbis virus and (ii) to change the pattern of intracellular viral RNA synthesis from that produced by infection with standard Sindbis virus alone. We conclude that defective interfering particles of Sindbis virus generated in chicken or hamster cells are silent or inert in mosquito cells.
虽然通过连续未稀释传代技术可在BHK - 21细胞或鸡胚成纤维细胞中轻易产生辛德毕斯病毒的缺陷干扰颗粒,但类似方法未能在白纹伊蚊细胞培养物中产生此类颗粒。此外,在BHK - 21细胞或鸡胚成纤维细胞中产生的、含有缺陷干扰颗粒的辛德毕斯病毒毒株,在白纹伊蚊细胞中进行测试时,(i)未能干扰标准辛德毕斯病毒的复制,(ii)也未能改变由单独感染标准辛德毕斯病毒所产生的细胞内病毒RNA合成模式。我们得出结论,在鸡或仓鼠细胞中产生的辛德毕斯病毒缺陷干扰颗粒在蚊细胞中是沉默的或无活性的。