Schreiter P P, Gillor O, Post A, Belkin S, Schmid R D, Bachmann T T
Institute of Technical Biochemistry, University of Stuttgart, Allmandring 31, D-70569 Stuttgart, Germany.
Biosens Bioelectron. 2001 Dec;16(9-12):811-8. doi: 10.1016/s0956-5663(01)00224-x.
Massive growth of cyanobacteria, known as "algal blooms", has become a major concern for water monitoring. It has been observed that environmental factors like temperature, light, and certain patterns of availability of nutrients such as P, N, Fe influence cyanobacterial proliferation and toxin production. In order to monitor nutrients in aquatic ecosystems, an assay for monitoring phosphorus bioavailability to cyanobacteria was developed. The test consists of an immobilized luminescent reporter strain of Synechococcus PCC 7942, designated APL. The reporter strain harbours the gene coding the reporter protein luciferase from Vibrio harveyi under control of the inducible alkaline phosphatase promoter from Synechococcus PCC 7942, and can be induced under phosphorus limitation. The resultant CyanoSensor detects PO(3-)(4)-P in a concentration range of 0.3-8 microM after a sample incubation time of 8 h under continuous illumination (50 microE m(-2) s(-1)). The sensor also responded to a variety of organic phosphorus sources and was storable for 3 weeks at 4 degrees C. It could be demonstrated that the CyanoSensor for bioavailability monitoring is an improvement to conventional phosphorus detection methods.
蓝藻的大量繁殖，即所谓的“藻华”，已成为水质监测的主要关注点。据观察，温度、光照等环境因素以及磷、氮、铁等某些营养物质的可利用模式会影响蓝藻的增殖和毒素产生。为了监测水生生态系统中的营养物质，开发了一种用于监测蓝藻对磷生物可利用性的检测方法。该测试由固定化的聚球藻PCC 7942发光报告菌株组成，命名为APL。该报告菌株在聚球藻PCC 7942的诱导型碱性磷酸酶启动子控制下，含有编码来自哈维弧菌的报告蛋白荧光素酶的基因，并且在磷限制条件下可被诱导。在连续光照（50 μE m⁻² s⁻¹）下样品孵育8小时后，所得的蓝藻传感器可检测浓度范围为0.3 - 8 μM的磷酸根离子（PO₄³⁻）。该传感器还对多种有机磷源有响应，并且在4℃下可储存3周。可以证明，用于生物可利用性监测的蓝藻传感器是对传统磷检测方法的一种改进。
