Department of Biological Sciences, Bowling Green State University, Bowling Green, OH, 43403, USA.
Adv Biochem Eng Biotechnol. 2010;118:165-88. doi: 10.1007/10_2009_23.
Due to their ubiquity in aquatic environments and their contribution to total biomass, especially in oligotrophic systems, cyanobacteria can be viewed as a proxy for primary productivity in both marine and fresh waters. In this chapter we describe the development and use of picocyanobacterial bioreporters to measure the bioavailability of nutrients that may constrain total photosynthesis in both lacustrine and marine systems. Issues pertaining to bioreporter construction, performance and field applications are discussed. Specifically, luminescent Synechococcus spp. and Synechocystis spp. bioreporters are described that allow the bioavailability of phosphorus, nitrogen and iron to be accurately measured in environmental samples.
由于蓝细菌在水生环境中的普遍存在及其对总生物量的贡献，特别是在贫营养系统中，它们可以被视为海洋和淡水初级生产力的替代物。在本章中，我们描述了利用微囊藻生物报告器来测量可能限制湖泊和海洋系统总光合作用的营养物质的生物可利用性的开发和应用。讨论了与生物报告器构建、性能和现场应用相关的问题。具体来说，描述了发光聚球藻属和集胞藻属生物报告器，可准确测量环境样品中磷、氮和铁的生物可利用性。
