Belkin Shimshon
Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 9190401, Israel.
Biosensors (Basel). 2025 Feb 14;15(2):111. doi: 10.3390/bios15020111.
This review attempts to summarize my three decades-long involvement in, and contribution to, the design, construction and testing of bioluminescent microbial sensor strains (bioreporters). With the understanding that such a document cannot be completely free of bias, the review focuses on studies from my own lab only, with almost no coverage of the parallel progress made by others in similar fields. This admittedly subjective approach by no way detracts from the achievements of countless excellent researchers who are not mentioned here, and whose contributions to the field are at least as important as that of my own. The review covers basic aspects of microbial sensor design, and then progresses to describe approaches to performance improvement of sensor strains aimed at the detection of either specific chemicals, groups of chemicals sharing similar characteristics, or global effects, such as toxicity and genotoxicity. The need for integration of live sensor cells into a compatible hardware platform is highlighted, as is the importance of long-term maintenance of the cells' viability and activity. The use of multi-member sensors' panels is presented as a means for enhancing the detection spectrum and sample "fingerprinting", along with a list of different purposes to which such sensors have been put to use.
本综述试图总结我三十年来在生物发光微生物传感器菌株（生物报告器）的设计、构建和测试方面的参与情况及所做贡献。鉴于这样一份文档不可能完全没有偏见，本综述仅聚焦于我自己实验室的研究，几乎没有涉及其他在类似领域取得的平行进展。诚然，这种主观的方法丝毫不减损无数未在此提及的优秀研究人员的成就，他们对该领域的贡献至少与我自己的一样重要。该综述涵盖了微生物传感器设计的基本方面，接着描述了旨在检测特定化学物质、具有相似特征的化学物质组或诸如毒性和遗传毒性等全局效应的传感器菌株性能改进方法。强调了将活的传感器细胞整合到兼容硬件平台的必要性，以及长期维持细胞活力和活性的重要性。还介绍了使用多成员传感器面板作为拓宽检测范围和进行样本“指纹识别”的一种手段，以及此类传感器已被用于的一系列不同目的。
