Chan L Y, Chiu P Y, Siu S S, Lau T K
Department of Obstetric and Gynaecology, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, Hong Kong.
Hum Reprod. 2001 Nov;16(11):2390-3. doi: 10.1093/humrep/16.11.2390.
Diclofenac is a non-steroidal anti-inflammatory drug, commonly used by reproductive age women for the treatment of a variety of conditions. However, there is limited information regarding the teratogenic effects of this drug.
The effect of diclofenac on the developing embryo during the critical period of organogenesis was investigated by using a whole rat embryo culture model. Embryos were exposed to various concentrations of diclofenac and scored for growth and differentiation at the end of the culture period.
Total developmental score and score for caudal neural tube, flexion and hindlimb were significantly lower in embryos exposed to high concentrations of diclofenac (7.5 and 15.0 microg/ml), but no difference in these parameters was observed when embryos were exposed to low concentration of diclofenac (1.5, 2.5 and 5.0 microg/ml). No significant differences in yolk sac diameter, crown-rump length and number of somites was found between embryos in the experimental and the control group.
Our study has demonstrated that diclofenac exerts direct teratogenic effects on rat embryos. Until more is known about the effects of diclofenac (especially in moderate to high doses) in women of reproductive age, we suggest its use should be treated with caution.
双氯芬酸是一种非甾体抗炎药，育龄妇女常用其治疗多种病症。然而，关于该药物致畸作用的信息有限。
通过使用全大鼠胚胎培养模型，研究双氯芬酸在器官发生关键期对发育中胚胎的影响。将胚胎暴露于不同浓度的双氯芬酸中，并在培养期结束时对其生长和分化进行评分。
暴露于高浓度双氯芬酸（7.5和15.0微克/毫升）的胚胎，其总发育评分以及尾神经管、屈曲和后肢评分显著较低，但当胚胎暴露于低浓度双氯芬酸（1.5、2.5和5.0微克/毫升）时，这些参数未观察到差异。实验组和对照组胚胎的卵黄囊直径、冠臀长度和体节数量均无显著差异。
我们的研究表明双氯芬酸对大鼠胚胎有直接致畸作用。在更多了解双氯芬酸（尤其是中高剂量）对育龄妇女的影响之前，我们建议谨慎使用该药。
