Yousem S A, Finkelstein S D, Swalsky P A, Bakker A, Ohori N P
Department of Pathology, University of Pittsburgh Medical Center-Presbyterian University Hospital, Pittsburgh, PA 15213-2582, USA.
Hum Pathol. 2001 Oct;32(10):1039-42. doi: 10.1053/hupa.2001.28249.
Bronchioloalveolar adenocarcinoma (BAC) morphologically resembles sheep pulmonary adenomatosis (SPA), a contagious ovine pulmonary adenocarcinoma caused by the jaagsiekte sheep retrovirus (JSRV). Previously, positivity for JSRV by immunostaining, reverse-transcription polymerase chain reaction (RT-PCR), and Western blot was reported in most nonmucinous BACs. Our objective in this study was to analyze additional BAC subtypes and conventional adenocarcinomas (CA) to further substantiate this association. Tumor tissue was microdissected from unstained paraffin sections of 26 cases of formalin-fixed, paraffin-embedded BAC (7 mucinous, 17 nonmucinous, 2 sclerosing) and 29 cases of CA. Positive controls consisted of 2 separate paraffin blocks of known SPA. Primer sequences were derived that were capable of hybridizing to all reported strain variants of both the DNA (endogenous) and RNA (exogenous) forms of JSRV. Each sample was tested using both PCR (DNA) and RT-PCR (RNA). All BAC and CA cases were negative for JSRV. Positive controls yielded PCR products that were sequenced and precisely matched the published prototype stain of JSRV. To control for negative effects of tissue fixation, dilutions of positive control tissue were added to BAC and CA samples. Detection of JSRV was evident at 1:50 dilution. Although the possibility of a viral association with BAC cannot be excluded, this study shows that the association with JSRV is probably very weak, if present at all.
细支气管肺泡腺癌（BAC）在形态上类似于绵羊肺腺瘤病（SPA），后者是一种由绵羊肺腺瘤逆转录病毒（JSRV）引起的传染性绵羊肺腺癌。此前，多数非黏液性BAC通过免疫染色、逆转录聚合酶链反应（RT-PCR）和蛋白质印迹法检测JSRV呈阳性。本研究的目的是分析更多BAC亚型和传统腺癌（CA），以进一步证实这种关联。从26例福尔马林固定、石蜡包埋的BAC（7例黏液性、17例非黏液性、2例硬化性）和29例CA的未染色石蜡切片中显微切割肿瘤组织。阳性对照由2个已知SPA的单独石蜡块组成。设计了能够与JSRV的DNA（内源性）和RNA（外源性）形式的所有报道菌株变体杂交的引物序列。每个样本均采用PCR（DNA）和RT-PCR（RNA）进行检测。所有BAC和CA病例的JSRV检测均为阴性。阳性对照产生的PCR产物经测序后与已发表的JSRV原型毒株精确匹配。为控制组织固定的负面影响，将阳性对照组织的稀释液添加到BAC和CA样本中。在1:50稀释度时可明显检测到JSRV。虽然不能排除病毒与BAC相关的可能性，但本研究表明，即使存在，与JSRV的关联可能也非常弱。
