通过高通量测序方法，在五例鳞屑状腺癌（原“细支气管肺泡癌”）中未发现病毒序列的证据。
No evidence for viral sequences in five lepidic adenocarcinomas (former "BAC") by a high-throughput sequencing approach.
作者信息
Berthet Nicolas, Frangeul Lionel, Olaussen Ken André, Brambilla Elisabeth, Dorvault Nicolas, Girard Philippe, Validire Pierre, Fadel Elie, Bouchier Christiane, Gessain Antoine, Soria Jean-Charles
机构信息
Epidemiology and Physiopathology of Oncogenic Viruses Unit, Institut Pasteur, 28 rue du Docteur Roux, 75015, Paris, France.
Centre National de la Recherche Scientifique, UMR 3569, 28 rue du Docteur Roux, 75015, Paris, France.
出版信息
BMC Res Notes. 2015 Dec 15;8:782. doi: 10.1186/s13104-015-1669-8.
BACKGROUND
The hypothesis of an infectious etiology of the formerly named bronchiolo-alveolar carcinoma (BAC) has raised controversy. We investigated tumor lung tissues from five patients with former BAC histology using high-throughput sequencing technologies to discover potential viruses present in this type of lung cancer. Around 180 million single reads of 100 bases were generated for each BAC sample.
RESULTS
None of the reads showed a significant similarity for Jaagsiekte sheep retrovirus (JSRV) and no other viruses were found except for endogenous retroviruses.
CONCLUSIONS
In conclusion, we have demonstrated the absence of JSRV and other known human viruses in five samples of well-characterized lepidic adenocarcinoma.
背景
既往命名为细支气管肺泡癌（BAC）的感染性病因假说引发了争议。我们使用高通量测序技术研究了5例具有既往BAC组织学特征患者的肿瘤肺组织，以发现这种类型肺癌中存在的潜在病毒。每个BAC样本产生了约1.8亿条100个碱基的单读长序列。
结果
没有读段显示与绵羊肺腺瘤病毒（JSRV）有显著相似性，除内源性逆转录病毒外未发现其他病毒。
结论
总之，我们已证实在5例特征明确的鳞屑状腺癌样本中不存在JSRV和其他已知人类病毒。
Zotero 插件