Newlin G E, Bussell R H
Arch Virol. 1975;47(2):97-107. doi: 10.1007/BF01320550.
A study of the mechanism of myxovirus and paramyxovirus inactivattion by hydroxylamine, O-methylhydroxylamine and N-methylhydroxylamine was conducted. Influenza A (WSN) was used as the sensitive myxovirus and Newcastle disease virus (NDV-L) was used as the relatively resistant paramyxovirus in certain experiments. Inactivation was found to be rapid (15 minutes) and mose effective at high concentrations (2M). All three compounds significantly decreased the hemagglutination titer of WSN treated at pH smaller than or equal to 5.0. There was no detectable change in NDV hemagglutination titer. Adsorption of hydroxylamine (pH 7.0) inactivated WSN appeared normal; however, the rate of adsorption was decreased when virus was inactivated by (pH 5.0) O-methylhydroxylamine. Equilibrium density gradient centrifugation in potassium tartrate showed no density changes in inactivated virus. WSN inactivated virus. WSN inactivated with 14C-O-methylhydroxylamine and subjected to RNA extraction showed greater than or equal to 35 percent of the 14C in the phenol phases and 21 per cent in the RNA. The 14C-O-methylhydroxylamine associated with the RNA of insensitive NDV was about 3 per cent of that associated with sensitive WSN-RNA. Hydroxylamine has no apparent effect on paramyxovirus (NDV) hemagglutination titer and less 14C-O-methylhydroxylamine is associated with the RNA of this virus. The results suggest these compounds may affect both the RNA and the envelope portion of myxoviruses (WSN) to produce inactivation.
开展了一项关于羟胺、O-甲基羟胺和N-甲基羟胺对黏液病毒和副黏液病毒灭活机制的研究。在某些实验中，甲型流感病毒（WSN）被用作敏感的黏液病毒，新城疫病毒（NDV-L）被用作相对耐药的副黏液病毒。发现灭活迅速（15分钟），且在高浓度（2M）时最有效。所有这三种化合物均能显著降低在pH小于或等于5.0条件下处理的WSN的血凝滴度。NDV的血凝滴度未检测到变化。羟胺（pH 7.0）对WSN的吸附灭活表现正常；然而，当病毒被（pH 5.0）O-甲基羟胺灭活时，吸附速率降低。酒石酸钾中的平衡密度梯度离心显示灭活病毒的密度没有变化。用14C-O-甲基羟胺灭活并进行RNA提取的WSN，在酚相中显示14C含量大于或等于35%，在RNA中为21%。与不敏感的NDV的RNA相关的14C-O-甲基羟胺约为与敏感的WSN-RNA相关的14C-O-甲基羟胺的3%。羟胺对副黏液病毒（NDV）的血凝滴度没有明显影响，且与该病毒的RNA相关的14C-O-甲基羟胺较少。结果表明，这些化合物可能会影响黏液病毒（WSN）的RNA和包膜部分以产生灭活作用。
