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通过质谱法鉴定蛋白质结合的碳水化合物。

Identification of protein-bound carbohydrates by mass spectrometry.

作者信息

Harvey D J

机构信息

Oxford Glycobiology Institute, Department of Biochemistry, South Parks Road, Oxford OXI 3QU, UK.

出版信息

Proteomics. 2001 Feb;1(2):311-28. doi: 10.1002/1615-9861(200102)1:2<311::AID-PROT311>3.0.CO;2-J.

DOI:10.1002/1615-9861(200102)1:2<311::AID-PROT311>3.0.CO;2-J
PMID:11680878
Abstract

This review outlines the use of modern methods of mass spectrometry, mainly based on electrospray ionization and matrix-assisted lased desorption/ionization, for the identification of protein-bound carbohydrates. The techniques are briefly described together with methods for glycan release and purification prior to mass spectrometry. Fragmentation of glycans, produced under various conditions, is described in the context of obtaining structural information at the sensitivity required for proteomic work.

摘要

本综述概述了主要基于电喷雾电离和基质辅助激光解吸/电离的现代质谱方法在鉴定蛋白质结合碳水化合物方面的应用。简要介绍了这些技术以及质谱分析前聚糖释放和纯化的方法。在蛋白质组学工作所需的灵敏度下获得结构信息的背景下，描述了在各种条件下产生的聚糖片段化情况。

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