Mucha Eike, Stuckmann Alexandra, Marianski Mateusz, Struwe Weston B, Meijer Gerard, Pagel Kevin
Fritz Haber Institute of the Max Planck Society , Department of Molecular Physics , Faradayweg 4-6 , 14195 Berlin , Germany . Email:
Institute of Chemistry and Biochemistry , Freie Universität Berlin , Takustraße 3 , 14195 Berlin , Germany.
Chem Sci. 2019 Jan 4;10(5):1272-1284. doi: 10.1039/c8sc05426f. eCollection 2019 Feb 7.
Although there have been substantial improvements in glycan analysis over the past decade, the lack of both high-resolution and high-throughput methods hampers progress in glycomics. This perspective article highlights the current developments of liquid chromatography, mass spectrometry, ion-mobility spectrometry and cryogenic IR spectroscopy for glycan analysis and gives a critical insight to their individual strengths and limitations. Moreover, we discuss a novel concept in which ion mobility-mass spectrometry and cryogenic IR spectroscopy is combined in a single instrument such that datasets consisting of /, collision cross sections and IR fingerprints can be obtained. This multidimensional data will then be compared to a comprehensive reference library of intact glycans and their fragments to accurately identify unknown glycans on a high-throughput scale with minimal sample requirements. Due to the complementarity of the obtained information, this novel approach is highly diagnostic and also suitable for the identification of larger glycans; however, the workflow and instrumentation is straightforward enough to be implemented into a user-friendly setup.
尽管在过去十年中聚糖分析取得了显著进展，但缺乏高分辨率和高通量方法阻碍了糖组学的发展。这篇观点文章重点介绍了用于聚糖分析的液相色谱、质谱、离子淌度光谱和低温红外光谱的当前发展情况，并对它们各自的优势和局限性进行了批判性洞察。此外，我们讨论了一个新颖的概念，即将离子淌度-质谱和低温红外光谱结合在一台仪器中，从而可以获得由迁移率、碰撞截面和红外指纹组成的数据集。然后，将这些多维数据与完整聚糖及其片段的综合参考库进行比较，以在高通量规模上以最少的样品需求准确鉴定未知聚糖。由于所获得信息的互补性，这种新方法具有高度的诊断性，也适用于鉴定更大的聚糖；然而，工作流程和仪器设备足够简单，可以实现用户友好的设置。
