Meade M J, Waddell R L, Callahan T M
Department of Biology, Allegheny College, 520 N. Main St., Meadville, PA 16335, USA.
FEMS Microbiol Lett. 2001 Oct 16;204(1):45-8. doi: 10.1111/j.1574-6968.2001.tb10860.x.
Triclosan is a broad-spectrum antimicrobial agent that has been incorporated into many household and medical products. Bacteria with high levels of triclosan resistance were isolated from compost, water, and soil samples. Two of these bacteria, Pseudomonas putida TriRY and Alcaligenes xylosoxidans subsp. denitrificans TR1, were able to use triclosan as a sole carbon source and clear particulate triclosan from agar. A decrease in triclosan concentration was measured by HPLC within 6 h of inoculation with strain TriRY and 24 h with strain TR1. Bioassays demonstrated that triclosan was inactivated in liquid cultures and/or embedded in plastic by the growth of strain TriRY and strain TR1, permitting the growth of triclosan-sensitive bacteria.
三氯生是一种广谱抗菌剂，已被添加到许多家用和医疗产品中。从堆肥、水和土壤样本中分离出了对三氯生具有高度抗性的细菌。其中两种细菌，恶臭假单胞菌TriRY和脱硝产碱杆菌木糖氧化亚种TR1，能够将三氯生用作唯一碳源，并从琼脂中清除颗粒状三氯生。用高效液相色谱法（HPLC）测定，接种TriRY菌株后6小时内以及接种TR1菌株后24小时内，三氯生浓度降低。生物测定表明，通过TriRY菌株和TR1菌株的生长，三氯生在液体培养物中失活和/或嵌入塑料中，从而使对三氯生敏感的细菌得以生长。
