Reinecke F, Groth T, Heise K P, Joentgen W, Müller N, Steinbüchel A
Institut für Mikrobiologie, Westfälische Wilhelms-Universität Münster, Germany.
FEMS Microbiol Lett. 2000 Jul 1;188(1):41-6. doi: 10.1111/j.1574-6968.2000.tb09166.x.
Three bacterial strains were isolated, which used the synthetic chelating agent iminodisuccinate (IDS) as sole carbon source for growth in mineral salts media (MSM). Taxonomic analysis and 16S rDNA sequence analysis identified one of these isolates (B3), which was isolated from sewage sludge, as a strain of Achromobacter xylosoxidans subsp. xylosoxidans. It exhibited a doubling time of approximately 3 h in liquid MSM supplemented with IDS and grew even in the presence of 1.0% (w/v) IDS. Since photometric and high performance liquid chromatography analysis showed that IDS, which came onto the market only recently as an alternative for ethylenediaminetetraacetate, was completely degraded by axenic cultures of bacteria; it will probably be readily degraded in the environment.
分离出了三株细菌菌株，它们在矿物盐培养基（MSM）中使用合成螯合剂亚氨基二琥珀酸（IDS）作为唯一碳源进行生长。分类学分析和16S rDNA序列分析确定，其中一株从污水污泥中分离出的菌株（B3）为木糖氧化无色杆菌木糖氧化亚种。在添加了IDS的液体MSM中，它的倍增时间约为3小时，甚至在存在1.0%（w/v）IDS的情况下也能生长。由于光度分析和高效液相色谱分析表明，作为乙二胺四乙酸替代品最近才上市的IDS被细菌的纯培养物完全降解；它在环境中可能很容易被降解。
