Ermakova I T, Safrina N S, Starovoĭtov I I, Liubun' E V, Shcherbakov A A, Makarov O E, Petrova A A, Shpil'kov P A
Mikrobiologiia. 2004 May-Jun;73(3):358-63.
Bacterial strains growing in medium with mustard gas reaction masses (RM) as carbon sources were obtained. Growth cessation in the above medium was caused by the exhaustion of bioutilizable substrates, first of all monoethanolamine (MEA) and ethyleneglycol (EG), rather than by the accumulation of toxic metabolites in the culture liquid or in the cells. The main RM components, 1,4-perhydrothiazines (PHT), formed in the course of chemical detoxication of mustard gas, were identified and analyzed. The predominant component of PHT mixture was N-(2-hydroxyethyl)-2-methyl-1,4-perhydrothiazine hydrochloride. Concentrations of all the PHT decreased by 50% in the course of culture growth; their destruction was a result of microbial metabolism.
获得了在含有芥子气反应产物（RM）作为碳源的培养基中生长的细菌菌株。上述培养基中生长停止是由于可生物利用底物的耗尽，首先是单乙醇胺（MEA）和乙二醇（EG），而不是由于培养液或细胞中有毒代谢产物的积累。对芥子气化学解毒过程中形成的主要RM成分1,4-全氢噻嗪（PHT）进行了鉴定和分析。PHT混合物的主要成分是N-(2-羟乙基)-2-甲基-1,4-全氢噻嗪盐酸盐。在培养生长过程中，所有PHT的浓度均下降了50%；它们的破坏是微生物代谢的结果。
