使用人乳头瘤病毒假病毒进行基因转移会因病毒基因型而异，并且需要细胞表面硫酸乙酰肝素。

Gene transfer using human papillomavirus pseudovirions varies according to virus genotype and requires cell surface heparan sulfate.

作者信息

Combita A L, Touzé A, Bousarghin L, Sizaret P Y, Muñoz N, Coursaget P

机构信息

Laboratoire de Virologie Moléculaire, INSERM EMIU 00-10, Faculté des Sciences Pharmaceutiques P. Maupas, 31 avenue Monge, 37200 Tours, France.

出版信息

FEMS Microbiol Lett. 2001 Oct 16;204(1):183-8. doi: 10.1111/j.1574-6968.2001.tb10883.x.

DOI:10.1111/j.1574-6968.2001.tb10883.x

PMID:11682199

Abstract

Artificial viruses consisting of DNA plasmid packaged in vitro into virus-like particles (VLPs) are new vehicles for gene transfer. We therefore investigated the ability of nine human papillomavirus (HPV) VLPs to interact with heterologous DNA and transfer genes. HPV 16, 18, 31, 33, 39, 45, 58, 59, and 68 VLPs were able to bind heterologous DNA and to transfer genes into Cos-7 cells. Inhibition of gene transfer by preincubation of the pseudovirions with heparin confirmed that heparan sulfate on the cell surface plays a role as cell receptor for HPVs. As HPV neutralizing antibodies are mainly type-specific, gene transfer with different HPV pseudovirions offers the possibility of their sequential use in vivo for a greater efficacy.

摘要

由体外包装在病毒样颗粒（VLP）中的DNA质粒组成的人工病毒是基因转移的新载体。因此，我们研究了九种人乳头瘤病毒（HPV）VLP与异源DNA相互作用并转移基因的能力。HPV 16、18、31、33、39、45、58、59和68 VLP能够结合异源DNA并将基因转移到Cos-7细胞中。用肝素预孵育假病毒对基因转移的抑制作用证实，细胞表面的硫酸乙酰肝素作为HPV的细胞受体发挥作用。由于HPV中和抗体主要是型特异性的，使用不同的HPV假病毒进行基因转移为它们在体内序贯使用以提高疗效提供了可能性。