通过抑制人乳头瘤病毒（HPV）假病毒介导的基因转移来检测抗人乳头瘤病毒（HPV）的中和抗体。
Detection of neutralizing antibodies against human papillomaviruses (HPV) by inhibition of gene transfer mediated by HPV pseudovirions.
作者信息
Bousarghin Latifa, Combita-Rojas Alba-Lucia, Touzé Antoine, El Mehdaoui Slimane, Sizaret Pierre-Yves, Bravo Maria-Mercedes, Coursaget Pierre
机构信息
Laboratoire de Virologie Moléculaire, INSERM EMIU 00-10, Tours, France.
出版信息
J Clin Microbiol. 2002 Mar;40(3):926-32. doi: 10.1128/JCM.40.3.926-932.2002.
Abstract
The goal of this study was to develop a human papillomavirus (HPV) neutralization assay using HPV pseudovirions generated in vitro. For this purpose, gene transfer efficiency of HPV virus-like particles (VLPs) was improved by using direct interaction between a reporter plasmid and the VLPs. Electron microscopic observation of the interaction between DNA molecules and VLPs revealed that VLPs always interact with a single DNA molecule and that VLPs bind to the end of linearized DNA molecules. An 100-fold improvement in the gene transfer was obtained by simple interaction between a linearized DNA molecule and VLPs. Moreover, direct interaction methods offer the possibility of transferring plasmids a size higher than that of the papillomavirus genome. The approach that we developed to generate HPV-16 and HPV-31 pseudovirions proved to be suitable for testing neutralizing antibodies in human sera both after immunization and after natural infection.
摘要
本研究的目的是利用体外产生的人乳头瘤病毒（HPV）假病毒开发一种HPV中和试验。为此，通过使用报告质粒与病毒样颗粒（VLP）之间的直接相互作用提高了HPV病毒样颗粒的基因转移效率。对DNA分子与VLP之间相互作用的电子显微镜观察显示，VLP总是与单个DNA分子相互作用，并且VLP与线性化DNA分子的末端结合。通过线性化DNA分子与VLP之间的简单相互作用，基因转移提高了100倍。此外，直接相互作用方法提供了转移大于乳头瘤病毒基因组大小的质粒的可能性。我们开发的产生HPV-16和HPV-31假病毒的方法被证明适用于检测免疫后和自然感染后人血清中的中和抗体。
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