Eun Alvin Jin-Cherng, Huang Liqun, Chew Fook Tim, Li Sam Fong-Yau, Wong Sek Man
Department of Biological Sciences, The National University of Singapore, Kent Ridge, 117543, Singapore, Singapore.
J Virol Methods. 2002 Jan;99(1-2):71-9. doi: 10.1016/s0166-0934(01)00382-2.
Quartz crystal microbalance (QCM) immunosensors are based on the principle that adsorption of substances on the surface of a quartz crystal changes its resonance oscillation frequency. A QCM immunosensor was developed for the detection of both cymbidium mosaic potexvirus (CymMV) and odontoglossum ringspot tobamovirus (ORSV) by pre-coating the QCMs with virus-specific antibodies. Upon binding of virions in either purified form or crude sap of infected orchids with the immobilised virus antibodies, the increase in mass at the QCM surface resulted in a reduction in the frequency of resonance oscillation in a manner dependent upon the amount of virus bound. The QCM was able to detect as low as 1 ng each of the two orchid viruses. This detection sensitivity is comparable to enzyme linked immunosorbent assay (ELISA) but the assay is faster. This immunoassay was shown to be specific, sensitive, rapid and economical, thus providing a viable alternative to virus detection methods. This is the first report using QCM immunosensors to detect plant viruses.
石英晶体微天平（QCM）免疫传感器基于物质吸附在石英晶体表面会改变其共振振荡频率这一原理。通过用病毒特异性抗体预涂覆QCM，开发了一种用于检测建兰花叶病毒（CymMV）和齿舌兰环斑病毒（ORSV）的QCM免疫传感器。当纯化形式的病毒粒子或感染兰花的粗汁液与固定化的病毒抗体结合时，QCM表面质量的增加导致共振振荡频率降低，其降低方式取决于结合的病毒量。QCM能够检测低至1纳克的两种兰花病毒。这种检测灵敏度与酶联免疫吸附测定（ELISA）相当，但检测速度更快。这种免疫测定方法被证明具有特异性、灵敏性、快速性和经济性，因此为病毒检测方法提供了一种可行的替代方案。这是首次使用QCM免疫传感器检测植物病毒的报告。
