Urinary histamine metabolite elevations during experimental influenza infection.

作者信息

Skoner D P, Gentile D A, Fireman P, Cordoro K, Doyle W J

机构信息

Department of Pediatrics, Children's Hospital of Pittsburgh, University of Pittsburgh School of Medicine, Pennsylvania 15213, USA.

出版信息

Ann Allergy Asthma Immunol. 2001 Oct;87(4):303-6. doi: 10.1016/s1081-1206(10)62244-2.

DOI:10.1016/s1081-1206(10)62244-2

PMID:11686422

Abstract

BACKGROUND

Although histamine is hypothesized to mediate symptoms induced by viral upper respiratory infections, elevations of this mediator have not been observed in nasal lavage fluids recovered from patients with viral upper respiratory infections.

OBJECTIVE

The purpose of this study was to use a novel method to determine whether histamine is released during experimental influenza A infection.

METHODS

Healthy adults (n = 15) were cloistered and inoculated intranasally with influenza A virus, and monitored for infection and illness. Daily morning void urines were collected and assayed for histamine and its metabolites by gas chromatography-mass spectrometry. Total histamine was calculated for each urine specimen by summing the assayed values of histamine and its metabolites.

RESULTS

All subjects were infected and developed illness. ANOVA documented a significant effect of study day (viral infection) on urinary levels of total histamine (P < 0.02). Pairwise analysis showed a significant elevation 2 days after inoculation.