一种来自椰子的抗真菌肽。
An antifungal peptide from the coconut.
作者信息
Wang H X, Ng T B
机构信息
Department of Microbiology, China Agricultural University and State Key Laboratory of Agrobiotechnology, Beijing, China.
出版信息
Peptides. 2005 Dec;26(12):2392-6. doi: 10.1016/j.peptides.2005.05.009.
A chromatographic procedure consisting of ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography on CM-cellulose, and gel filtration by fast performance liquid chromatography on Supedex 75 was utilized to isolate a 10 kDa antifungal peptide from coconut flesh. The peptide was unadsorbed on DEAE-cellulose, but adsorbed on Affi-gel blue gel and CM-cellulose. It displayed antifungal activity against Fusarium oxysporum, Mycosphaerella arachidicola and Physalospora piricola. The IC50 values of its inhibitory activities on mycelial growth in M. arachidicola and HIV-1 reverse transcriptase activity were respectively 1.2 and 52.5 microM.
采用一种色谱方法从椰肉中分离出一种10 kDa的抗真菌肽，该方法包括在DEAE-纤维素上进行离子交换色谱、在Affi-凝胶蓝凝胶上进行亲和色谱、在CM-纤维素上进行离子交换色谱以及在Superdex 75上通过快速高效液相色谱进行凝胶过滤。该肽不吸附于DEAE-纤维素，但吸附于Affi-凝胶蓝凝胶和CM-纤维素。它对尖孢镰刀菌、花生球腔菌和梨孢盘菌具有抗真菌活性。其对花生球腔菌菌丝体生长抑制活性的IC50值和对HIV-1逆转录酶活性的IC50值分别为1.2 microM和52.5 microM。
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