编码具有可卡因内影像的Ab2β抗独特型单克隆抗体重链和轻链可变区的cDNA的克隆与序列分析

Cloning and sequence analysis of cDNAs encoding the heavy and light chain variable regions of an Ab2beta anti-idiotypic monoclonal antibody possessing an internal image of cocaine.

作者信息

Ho M, Segre M

机构信息

Department of Pathobiology, University of Illinois, 2001 South Lincoln Ave, Urbana, IL 61802, USA.

出版信息

Biochim Biophys Acta. 2001 Oct 31;1521(1-3):135-40. doi: 10.1016/s0167-4781(01)00253-6.

DOI:10.1016/s0167-4781(01)00253-6

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3398996/

Abstract

We report here the cloning and sequence analysis of cDNAs encoding the variable regions of an Ab2beta anti-idiotypic monoclonal antibody (K1-4c, gamma1kappa) that mimics the configuration of cocaine. The Ab2beta specifically binds to the human dopamine transporter as shown by confocal immunofluorescence microscopy. The sequence of the heavy chain complementarity-determining region 3 of K1-4c is strikingly similar to that of a monoclonal antibody (F11.2.32) specific for HIV-1 protease. Three or four amino acids in the epitope recognized by the anti-HIV-1 protease antibody are also present in the third extracellular loop of the dopamine transporter. This epitope is within the conserved region of the known transporters for dopamine, norepinephrine and serotonin in Homo sapiens, Rattus norvegicus, Caenorhabditis elegans and Drosophila melanogaster.

摘要

我们在此报告编码模拟可卡因构型的Ab2β抗独特型单克隆抗体（K1-4c，γ1κ）可变区的cDNA的克隆及序列分析。共聚焦免疫荧光显微镜显示，Ab2β特异性结合人多巴胺转运体。K1-4c重链互补决定区3的序列与一种针对HIV-1蛋白酶的单克隆抗体（F11.2.32）的序列惊人地相似。抗HIV-1蛋白酶抗体识别的表位中的三或四个氨基酸也存在于多巴胺转运体的第三个细胞外环中。该表位位于人、大鼠、秀丽隐杆线虫和黑腹果蝇中已知的多巴胺、去甲肾上腺素和5-羟色胺转运体的保守区域内。

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