对一种具有乙肝表面抗原特异性的人单克隆抗体重链和轻链可变区编码cDNA的克隆及序列分析

Cloning and sequence analysis of cDNAs encoding the heavy and light chain variable regions of a human monoclonal antibody with specificity for hepatitis B surface antigen.

作者信息

Ryu C J, Chung H K, Hong H J

机构信息

Antibody Engineering Research Unit, Korea Research Institute of Bioscience and Biotechnology, Taejon 305-600, South Korea.

出版信息

Biochim Biophys Acta. 1998 Apr 10;1380(2):151-5. doi: 10.1016/s0304-4165(98)00006-3.

DOI:10.1016/s0304-4165(98)00006-3

Abstract

We cloned and analyzed the cDNA sequences encoding the variable regions of a human monoclonal antibody (23HN) (gamma1, kappa) that binds to the common a antigenic determinant on hepatitis B surface antigen and its fine epitope is different from those of previously known mAbs with the same specificity. The heavy and light chain variable regions are the members of human heavy chain subgroup III and kappa light chain subgroup III, respectively, and are most homologous to human germline VH segment DP-50 and Vkappa segment DPK-22, respectively.

摘要

我们克隆并分析了编码一种人单克隆抗体（23HN）（γ1，κ）可变区的cDNA序列，该抗体可结合乙型肝炎表面抗原上的共同a抗原决定簇，其精细表位与先前已知的具有相同特异性的单克隆抗体不同。重链和轻链可变区分别是人重链亚群III和κ轻链亚群III的成员，并且分别与人胚系VH区段DP-50和Vκ区段DPK-22最为同源。

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