维涅兰德固氮菌的非偶联NADH：泛醌氧化还原酶是高氧浓度下固氮生长所必需的。
Noncoupled NADH:ubiquinone oxidoreductase of Azotobacter vinelandii is required for diazotrophic growth at high oxygen concentrations.
作者信息
Bertsova Y V, Bogachev A V, Skulachev V P
机构信息
Department of Bioenergetics, A. N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow 119899, Russia.
出版信息
J Bacteriol. 2001 Dec;183(23):6869-74. doi: 10.1128/JB.183.23.6869-6874.2001.
Abstract
The gene encoding the noncoupled NADH:ubiquinone oxidoreductase (NDH II) from Azotobacter vinelandii was cloned, sequenced, and used to construct an NDH II-deficient mutant strain. Compared to the wild type, this strain showed a marked decrease in respiratory activity. It was unable to grow diazotrophically at high aeration, while it was fully capable of growth at low aeration or in the presence of NH(4)(+). This result suggests that the role of NDH II is as a vital component of the respiratory protection mechanism of the nitrogenase complex in A. vinelandii. It was also found that the oxidation of NADPH in the A. vinelandii respiratory chain is catalyzed solely by NDH II.
摘要
克隆、测序了编码来自棕色固氮菌的非偶联NADH：泛醌氧化还原酶（NDH II）的基因，并用于构建NDH II缺陷型突变菌株。与野生型相比，该菌株的呼吸活性显著降低。它在高通气条件下不能进行固氮生长，而在低通气条件下或存在NH₄⁺时能够完全生长。这一结果表明，NDH II的作用是作为棕色固氮菌中固氮酶复合物呼吸保护机制的重要组成部分。还发现棕色固氮菌呼吸链中NADPH的氧化仅由NDH II催化。
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