Iba K, Chiba H, Yamashita T, Ishii S, Sawada N
Department of Orthopaedic Surgery, Sapporo Medical University School of Medicine, Japan.
Cell Struct Funct. 2001 Aug;26(4):227-33. doi: 10.1247/csf.26.227.
We have recently reported that retinoic acid inhibits dexamethasone-induced alkaline phosphatase activity and mineralization in human osteoblastic cell line SV-HFO. In this study, we show that this inhibitory effect on alkaline phosphatase activity depends on the stage of cell differentiation; however, expression of tetranectin, which is a recently reported bone matrix protein, was completely inhibited by treatment with retinoic acid, irrespective of the stage of cell differentiation. Similarly, mineral deposit formation in SV-HFO cells was phase-independently inhibited by retinoic acid. To our knowledge, this is the first report that retinoic acid downregulates the tetranectin expression in human osteoblastic cells independent of the stage of cell differentiation, and is correlated with inhibition of mineralization.
我们最近报道，视黄酸可抑制人成骨细胞系SV-HFO中地塞米松诱导的碱性磷酸酶活性和矿化作用。在本研究中，我们发现这种对碱性磷酸酶活性的抑制作用取决于细胞分化阶段；然而，四连蛋白（一种最近报道的骨基质蛋白）的表达，无论细胞分化处于何种阶段，均被视黄酸处理完全抑制。同样，视黄酸对SV-HFO细胞中的矿物质沉积形成具有阶段非依赖性抑制作用。据我们所知，这是第一份关于视黄酸在不依赖细胞分化阶段的情况下下调人成骨细胞中四连蛋白表达，并与矿化抑制相关的报道。
