Iba K, Sawada N, Chiba H, Wewer U M, Ishii S, Mori M
Department of Pathology, Sapporo Medical University School of Medicine, Japan.
FEBS Lett. 1995 Oct 2;373(1):1-4. doi: 10.1016/0014-5793(95)00992-i.
In the present study, we examined the regulation of tetranectin gene expression using a human osteoblastic cell line, SV-HFO, that undergoes mineralization upon treatment with dexamethasone. We found that the expression of tetranectin and alkaline phosphatase mRNA was induced by dexamethasone treatment as evidenced by Northern blotting. When transforming growth factor-beta 1 (TGF-beta 1) was added together with dexamethasone to the SV-HFO cell cultures, the mineralization process was markedly suppressed and the expression of tetra nectin and alkaline phosphatase was downregulated in a dose-dependent manner. These results demonstrate that the expression of tetranectin in these osteoblastic cells is regulated by dexamethasone and TGF-beta 1 and that tetranectin expression is tightly linked to the process of mineralization.
在本研究中，我们使用人成骨细胞系SV-HFO研究了骨粘连蛋白基因表达的调控，该细胞系在用地塞米松处理后会发生矿化。我们发现，用地塞米松处理可诱导骨粘连蛋白和碱性磷酸酶mRNA的表达，Northern印迹法证实了这一点。当将转化生长因子-β1（TGF-β1）与地塞米松一起添加到SV-HFO细胞培养物中时，矿化过程受到明显抑制，骨粘连蛋白和碱性磷酸酶的表达以剂量依赖的方式下调。这些结果表明，这些成骨细胞中骨粘连蛋白的表达受地塞米松和TGF-β1的调控，并且骨粘连蛋白的表达与矿化过程紧密相关。
