Bisby R H, Cundall R B, Wardman P
Biochim Biophys Acta. 1975 Apr 21;389(1):137-44. doi: 10.1016/0005-2736(75)90391-0.
The reactions of the free radicals eaq- minus, OH and Br2- minus with haemoglobin-free erythrocyte ghost membranes have been studied by producing the radicals by pulse radiolysis and monitoring their reactions by optical spectroscopy. Hydrated electrons react rapidly with the membrane, but no attack at disulphide links was observed. Hydroxyl radical attack produced transient species absorbing weakly in the ultraviolet, which may arise from carbohydrate residues, such as N-acetyl neuraminic acid and N-acetyl glucosamine, on the membrane surface. No evidence was obtained for OH attack at ring-containing amino acid residues of the protein component. The Br2- minus radical, a more selective electrophile than OH, reacted only slowly with erythrocyte ghosts. Solubilization of the membranes with dodecylsulphate or digestion with alkali exposed protein containing tyrosine and tryptophan residues which reacted with Br2- minus. These results support other evidence for the absence of reactive protein at the membrane surface.
通过脉冲辐解产生自由基并利用光学光谱监测其反应，研究了水合电子（eaq⁻）、羟基自由基（OH）和溴负离子自由基（Br₂⁻）与无血红蛋白的红细胞血影膜的反应。水合电子与膜快速反应，但未观察到对二硫键的攻击。羟基自由基攻击产生了在紫外区域有微弱吸收的瞬态物种，这可能源于膜表面的碳水化合物残基，如N - 乙酰神经氨酸和N - 乙酰葡糖胺。未获得羟基自由基攻击蛋白质组分中含环氨基酸残基的证据。溴负离子自由基是比羟基自由基更具选择性的亲电试剂，它与红细胞血影反应缓慢。用十二烷基硫酸盐使膜增溶或用碱消化后，暴露出含有与溴负离子自由基反应的酪氨酸和色氨酸残基的蛋白质。这些结果支持了膜表面不存在反应性蛋白质的其他证据。
