从狒狒中分离和鉴定一种内源性巨细胞病毒（BaCMV）。

Isolation and characterization of an endogenous cytomegalovirus (BaCMV) from baboons.

作者信息

Blewett E L, White G, Saliki J T, Eberle R

机构信息

Department of Biochemistry and Microbiology, College of Osteopathic Medicine, Oklahoma State University, Tulsa 74107, USA.

出版信息

Arch Virol. 2001;146(9):1723-38. doi: 10.1007/s007050170059.

DOI:10.1007/s007050170059

PMID:11699958

Abstract

This report describes the isolation of CMV-like viruses from olive, yellow and chacma sub-species of baboons. The viruses were identified as CMVs by their characteristic growth properties in cell culture, virion morphology under the TEM, and antigenic cross-reactivity with other primate CMVs. The glycoprotein B gene homologue from an olive baboon CMV isolate (BaCMV OCOM4-37) was identified, cloned and sequenced. We present the sequence of this gene and by phylogenetic analysis demonstrate that BaCMV is in fact a cytomegalovirus, and is more closely related to rhesus CMV than to human CMV. An ELISA was developed to measure anti-BaCMV antibodies in baboon sera. Serological testing of colony-bred and wild-born baboons indicated that BaCMV is ubiquitous in all baboon populations, with >95% of adult baboons of all sub-species being infected.

摘要

本报告描述了从狒狒的橄榄色亚种、黄色亚种和南非大狒狒亚种中分离出类巨细胞病毒（CMV）。通过这些病毒在细胞培养中的特征性生长特性、透射电子显微镜下的病毒粒子形态以及与其他灵长类CMV的抗原交叉反应性，将其鉴定为CMV。鉴定、克隆并测序了来自橄榄色狒狒CMV分离株（BaCMV OCOM4 - 37）的糖蛋白B基因同源物。我们展示了该基因的序列，并通过系统发育分析证明BaCMV实际上是一种巨细胞病毒，与恒河猴CMV的亲缘关系比与人类CMV更近。开发了一种酶联免疫吸附测定法（ELISA）来检测狒狒血清中的抗BaCMV抗体。对圈养繁殖和野生出生的狒狒进行的血清学检测表明，BaCMV在所有狒狒种群中普遍存在，所有亚种中超过95%的成年狒狒受到感染。