Wingard L B, Schiller J G, Wolfson S K, Liu C C, Drash A L, Yao S J
J Biomed Mater Res. 1979 Nov;13(6):921-35. doi: 10.1002/jbm.820130610.
Glucose oxidase, catalase, and bovine serum albumin were co-immobilized with glutaraldehyde around a platinum screen or around a single platinum-iridium wire. The potential difference between this dual enzyme electrode and a Ag/AgCL reference electrode was proportional to the logarithm of the glucose concentration over the range from 10 to about 150 mg glucose per 100 ml in buffered solution at pH 7.4 and 37 degrees C. The enzyme electrode responded in serum only if coated with a semipermeable film, such as cellulose acetate, to exclude serum macromolecules. The potentiometric results were similar to those obtained with the two enzymes co-immobilized in polyacrylamide gel around a platinum screen or with only one of the enzymes, glucose oxidase, covalently coupled to a platinum screen. The results so far suggest that glucose for development of a continuous in vivo glucose sensor.
葡萄糖氧化酶、过氧化氢酶和牛血清白蛋白通过戊二醛共固定在铂丝网或单根铂铱丝周围。在pH 7.4、37摄氏度的缓冲溶液中，这种双酶电极与Ag/AgCl参比电极之间的电位差与葡萄糖浓度的对数成正比，葡萄糖浓度范围为每100毫升10至约150毫克。只有在涂覆半透膜（如醋酸纤维素）以排除血清大分子时，酶电极才会在血清中产生响应。电位测定结果与在铂丝网周围的聚丙烯酰胺凝胶中共固定两种酶或仅将其中一种酶（葡萄糖氧化酶）共价偶联到铂丝网上所获得的结果相似。目前的结果表明，葡萄糖可用于开发连续的体内葡萄糖传感器。
