Wingard L B, Liu C C, Wolfson S K, Yao S J, Drash A L
Diabetes Care. 1982 May-Jun;5(3):199-202. doi: 10.2337/diacare.5.3.199.
A series of enzyme electrodes for measurement of glucose have been constructed. The electrodes contain glucose oxidase immobilized on platinum, either with or without co-immobilization of catalase. When placed in buffered glucose, the enzyme electrodes show a potentiometric response to glucose with respect to a Ag/AgCl reference electrode. This response is reproducible in the physiologic range of glucose concentrations. The immobilization technique, some of the environmental variables such as oxygen concentration and pH, and several compounds that might interfere with the selectivity of the enzyme electrodes for glucose have received preliminary study. This direct potentiometric approach is undergoing further evaluation to determine the basic electrochemical mechanism responsible for the potentiometric signal and whether it can be adapted for continuous in vivo monitoring of the glucose concentration in body fluids.
已经构建了一系列用于测量葡萄糖的酶电极。这些电极包含固定在铂上的葡萄糖氧化酶，有或没有共固定过氧化氢酶。当置于缓冲葡萄糖溶液中时，酶电极相对于Ag/AgCl参比电极对葡萄糖呈现电位响应。这种响应在葡萄糖浓度的生理范围内是可重复的。固定技术、一些环境变量如氧浓度和pH值，以及几种可能干扰酶电极对葡萄糖选择性的化合物都已得到初步研究。这种直接电位法正在进一步评估，以确定产生电位信号的基本电化学机制，以及它是否可适用于连续体内监测体液中的葡萄糖浓度。
