Torchia E C, Labonté E D, Agellon L B
Department of Biochemistry and Canadian Institutes of Health Research Group in Molecular and Cell Biology of Lipids, University of Alberta, Edmonton, Alberta, T6G 2S2, Canada.
Anal Biochem. 2001 Nov 15;298(2):293-8. doi: 10.1006/abio.2001.5379.
We developed a quantitative method for the analysis of bile acids using a high performance liquid chromatograph coupled to an evaporative light scattering detector. An isocratic solvent system was used to resolve in a single run conjugated and unconjugated bile acid species relevant in human and rodent physiology. The detection of various bile acids was linear over a range of 0.08 to 10 nmol of injected molecules. The developed system is a convenient and cost-effective method for the routine analysis of a wide variety of bile acids.
我们开发了一种使用高效液相色谱仪与蒸发光散射检测器联用的胆汁酸定量分析方法。采用等度溶剂系统在一次运行中分离出与人类和啮齿动物生理学相关的结合型和非结合型胆汁酸种类。各种胆汁酸的检测在注入分子0.08至10 nmol的范围内呈线性。所开发的系统是一种用于常规分析多种胆汁酸的便捷且经济高效的方法。
