Bala S, Uniyal G C, Dubey T, Singh S P
Central Institute of Medicinal and Aromatic Plants (CIMAP), P.O. CIMAP, Lucknow-226015, India.
Phytochem Anal. 2001 Jul-Aug;12(4):277-80. doi: 10.1002/pca.586.
A reversed-phase column liquid chromatographic method for the analysis of sennosides A and B present in leaf and pod extracts of Cassia angustifolia has been developed using a Symmetry C18 column and a linear binary gradient profile. The method can be utilised for the quantitative determination of other sennosides as a baseline resolution for most of the constituents was achieved. The method is economical in terms of the time taken and the amount of solvent used (25 mL) for each analysis. The validity of the method with respect to analysis was confirmed by comparing the UV spectra of each peak with those of reference compounds using a photodiode array detector.
已开发出一种反相柱液相色谱法，用于分析狭叶番泻叶和荚果提取物中的番泻苷A和B，该方法使用Symmetry C18柱和线性二元梯度洗脱。由于大多数成分实现了基线分离，该方法可用于其他番泻苷的定量测定。该方法在每次分析所需时间和所用溶剂量（25 mL）方面较为经济。通过使用光电二极管阵列检测器将每个峰的紫外光谱与参考化合物的光谱进行比较，证实了该分析方法的有效性。
