Sun Yan, Li Xuetuo, Yu Xingju
Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116023, China.
Se Pu. 2004 Jan;22(1):48-50.
A method for the separation and determination of sennosides A and B and the main composition (sennidins A and B) in degraded products of sennosides by linear gradient high performance liquid chromatography has been developed. Separation conditions were as follows: column, a Spherisorb C18 column (250 mm x 4.6 mm i.d., 10 microm); column temperature, 40 degrees C; detection wavelength, 360 nm; mobile phase A, 1.25% acetic acid aqueous solution; mobile phase B, methanol; linear gradient, 100% A --> (20 min) 100% B. The method is effective, quick, accurate and reproducible. The satisfactory results show that this new method has certain practical values as an approach of real-time analysis in the process of sennoside metabolism.
建立了一种采用线性梯度高效液相色谱法分离和测定番泻苷A和B及其降解产物中的主要成分（番泻二蒽酮A和B）的方法。分离条件如下：色谱柱为Spherisorb C18柱（250 mm×4.6 mm内径，10μm）；柱温40℃；检测波长360 nm；流动相A为1.25%乙酸水溶液；流动相B为甲醇；线性梯度为100%A→（20分钟）100%B。该方法高效、快速、准确且具有重现性。令人满意的结果表明，作为番泻苷代谢过程中实时分析的一种方法，这种新方法具有一定的实用价值。
