实验性感染的泌乳猫乳汁中弓形虫的检测。

Detection of Toxoplasma gondii in the milk of experimentally infected lactating cats.

作者信息

Powell C C, Brewer M, Lappin M R

机构信息

Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado, CO 80523, USA.

出版信息

Vet Parasitol. 2001 Dec 3;102(1-2):29-33. doi: 10.1016/s0304-4017(01)00521-0.

DOI:10.1016/s0304-4017(01)00521-0

PMID:11705649

Abstract

Tachyzoites of Toxoplasma gondii have been found in the milk of sheep, goats, cows and mice and infection by ingestion of raw goat milk has been documented in humans. Lactational transmission from infected cats to their kittens is suspected but the organism has not been detected in the milk. The purpose of this study was to demonstrate the presence of T. gondii in the milk of experimentally infected cats. Pregnant specific pathogen free cats were inoculated orally with T. gondii at various times prior to parturition. Feces were examined for oocyst shedding after sugar solution centrifugation. Milk was collected for polymerase chain reaction (PCR) and bioassay in mice. T. gondii was detected in the milk of five of six cats by either bioassay or PCR.

摘要

在绵羊、山羊、奶牛和小鼠的乳汁中发现了刚地弓形虫速殖子，并且已有文献记载人类因摄入生山羊奶而感染。怀疑存在从受感染的猫通过哺乳传染给小猫的情况，但尚未在乳汁中检测到该病原体。本研究的目的是证明实验感染的猫的乳汁中存在刚地弓形虫。在分娩前的不同时间给怀孕的无特定病原体猫经口接种刚地弓形虫。糖水离心后检查粪便中的卵囊排出情况。收集乳汁用于聚合酶链反应（PCR）和小鼠生物测定。通过生物测定或PCR在六只猫中的五只猫的乳汁中检测到了刚地弓形虫。