Hecht V, Vielle-Calzada J P, Hartog M V, Schmidt E D, Boutilier K, Grossniklaus U, de Vries S C
Laboratory of Molecular Biology, Wageningen University, 6703HA Wageningen, The Netherlands.
Plant Physiol. 2001 Nov;127(3):803-16.
We report here the isolation of the Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE 1 (AtSERK1) gene and we demonstrate its role during establishment of somatic embryogenesis in culture. The AtSERK1 gene is highly expressed during embryogenic cell formation in culture and during early embryogenesis. The AtSERK1 gene is first expressed in planta during megasporogenesis in the nucellus [corrected] of developing ovules, in the functional megaspore, and in all cells of the embryo sac up to fertilization. After fertilization, AtSERK1 expression is seen in all cells of the developing embryo until the heart stage. After this stage, AtSERK1 expression is no longer detectable in the embryo or in any part of the developing seed. Low expression is detected in adult vascular tissue. Ectopic expression of the full-length AtSERK1 cDNA under the control of the cauliflower mosaic virus 35S promoter did not result in any altered plant phenotype. However, seedlings that overexpressed the AtSERK1 mRNA exhibited a 3- to 4-fold increase in efficiency for initiation of somatic embryogenesis. Thus, an increased AtSERK1 level is sufficient to confer embryogenic competence in culture.
我们在此报告拟南芥体细胞胚胎发生受体类激酶1（AtSERK1）基因的分离，并证明其在培养中体细胞胚胎发生建立过程中的作用。AtSERK1基因在培养中的胚性细胞形成过程以及早期胚胎发生过程中高度表达。AtSERK1基因最初在发育中胚珠珠心（已修正）的大孢子发生过程中、功能大孢子中以及直至受精前胚囊的所有细胞中在植物体内表达。受精后，在发育中胚胎的所有细胞中直至心形期都能看到AtSERK1的表达。在此阶段之后，在胚胎或发育中种子的任何部分都检测不到AtSERK1的表达。在成年维管组织中检测到低水平表达。在花椰菜花叶病毒35S启动子控制下的全长AtSERK1 cDNA的异位表达并未导致任何植物表型改变。然而，过表达AtSERK1 mRNA的幼苗在体细胞胚胎发生起始效率上提高了3至4倍。因此，AtSERK1水平的提高足以赋予培养中的胚性能力。
