Lewinsohn E, Schalechet F, Wilkinson J, Matsui K, Tadmor Y, Nam K H, Amar O, Lastochkin E, Larkov O, Ravid U, Hiatt W, Gepstein S, Pichersky E
Newe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel.
Plant Physiol. 2001 Nov;127(3):1256-65.
The aromas of fruits, vegetables, and flowers are mixtures of volatile metabolites, often present in parts per billion levels or less. We show here that tomato (Lycopersicon esculentum Mill.) plants transgenic for a heterologous Clarkia breweri S-linalool synthase (LIS) gene, under the control of the tomato late-ripening-specific E8 promoter, synthesize and accumulate S-linalool and 8-hydroxylinalool in ripening fruits. Apart from the difference in volatiles, no other phenotypic alterations were noted, including the levels of other terpenoids such as gamma- and alpha-tocopherols, lycopene, beta-carotene, and lutein. Our studies indicate that it is possible to enhance the levels of monoterpenes in ripening fruits by metabolic engineering.
水果、蔬菜和花卉的香气是挥发性代谢物的混合物，其含量通常处于十亿分之一或更低水平。我们在此表明，在番茄晚熟特异性E8启动子的控制下，转异源布鲁氏克拉克花S-芳樟醇合酶（LIS）基因的番茄（Lycopersicon esculentum Mill.）植株在成熟果实中合成并积累S-芳樟醇和8-羟基芳樟醇。除了挥发性物质的差异外，未观察到其他表型变化，包括其他萜类化合物如γ-和α-生育酚、番茄红素、β-胡萝卜素和叶黄素的水平。我们的研究表明，通过代谢工程提高成熟果实中单萜类物质的水平是可能的。
