Departamento de Biología Vegetal, Universidad de Valencia, Av. Vicent Andrés Estellés S/N, Burjasot, 46100 Valencia, Spain; ISIC/ERI de Biotecnología y Biomedicina, Universidad de Valencia, Av. Vicent Andrés Estellés S/N, Burjasot, 46100 Valencia, Spain.
Instituto de Biología Molecular y Celular de Plantas, Departamento Biotecnología, Universidad Politécnica de Valencia-Consejo Superior de Investigaciones Científicas, C/Ingeniero Fausto Elio, 46022 Valencia, Spain.
Metab Eng. 2014 May;23:136-44. doi: 10.1016/j.ymben.2014.03.003. Epub 2014 Mar 28.
Transgenic Lavandula latifolia plants overexpressing the linalool synthase (LIS) gene from Clarkia breweri, encoding the LIS enzyme that catalyzes the synthesis of linalool were generated. Most of these plants increased significantly their linalool content as compared to controls, especially in the youngest leaves, where a linalool increase up to a 1000% was observed. The phenotype of increased linalool content observed in young leaves was maintained in those T1 progenies that inherit the LIS transgene, although this phenotype was less evident in the flower essential oil. Cross-pollination of transgenic spike lavender plants allowed the generation of double transgenic plants containing the DXS (1-deoxy-d-xylulose-5-P synthase), coding for the first enzyme of the methyl-d-erythritol-4-phosphate pathway, and LIS genes. Both essential oil yield and linalool content in double DXS-LIS transgenic plants were lower than that of their parentals, which could be due to co-suppression effects linked to the structures of the constructs used.
生成了过表达来自 Clarkia breweri 的芳樟醇合酶（LIS）基因的转化薰衣草植物，该基因编码催化芳樟醇合成的 LIS 酶。与对照相比，这些植物中的大多数的芳樟醇含量显著增加，特别是在最年轻的叶片中，观察到芳樟醇增加了 1000%。在继承 LIS 转基因的 T1 后代中，观察到的年轻叶片中增加的芳樟醇含量的表型得以维持，尽管这种表型在花精油中不太明显。转基因穗薰衣草植物的异花授粉允许生成含有 DXS（1-脱氧-d-木酮糖-5-P 合酶）的双转基因植物，该基因编码甲基-d-赤藓醇-4-磷酸途径的第一个酶，以及 LIS 基因。双 DXS-LIS 转基因植物的精油产量和芳樟醇含量均低于其亲本，这可能是由于与所使用的构建体结构相关的共抑制效应所致。
