Phan T T, See P, Lee S T, Chan S Y
Department of Plastic Surgery/National Burns Centre, Singapore General Hospital.
J Trauma. 2001 Nov;51(5):927-31. doi: 10.1097/00005373-200111000-00017.
Curcumin, isolated from turmeric, has been known to possess many pharmacologic properties. It has been proven to exhibit remarkable anticarcinogenic, anti-inflammatory, and antioxidant properties. Turmeric curcumin may be a good potential agent for wound healing.
To further understand its therapeutic mechanisms on wound healing, the antioxidant effects of curcumin on hydrogen peroxide (H2O2) and hypoxanthine-xanthine oxidase induced damage to cultured human keratinocytes and fibroblasts were investigated. Cell viability was assessed by colorimetric assay and quantification of lactate dehydrogenase release.
Exposure of human keratinocytes to curcumin at 10 microg/mL showed significant protective effect against hydrogen peroxide. Interestingly, exposure of human dermal fibroblasts to curcumin at 2.5 microg/mL showed significant protective effects against hydrogen peroxide. No protective effects of curcumin on either fibroblasts or keratinocytes against hypoxanthine-xanthine oxidase induced damage were found in our present studies.
The findings indicate that curcumin indeed possessed powerful inhibition against hydrogen peroxide damage in human keratinocytes and fibroblasts.
从姜黄中分离出的姜黄素具有多种药理特性。已证实其具有显著的抗癌、抗炎和抗氧化特性。姜黄中的姜黄素可能是一种良好的伤口愈合潜在药物。
为进一步了解其在伤口愈合中的治疗机制，研究了姜黄素对过氧化氢（H2O2）以及次黄嘌呤 - 黄嘌呤氧化酶诱导的培养人角质形成细胞和成纤维细胞损伤的抗氧化作用。通过比色法和乳酸脱氢酶释放量的定量来评估细胞活力。
人角质形成细胞暴露于10微克/毫升的姜黄素时，对过氧化氢显示出显著的保护作用。有趣的是，人真皮成纤维细胞暴露于2.5微克/毫升的姜黄素时，对过氧化氢也显示出显著的保护作用。在我们目前的研究中，未发现姜黄素对成纤维细胞或角质形成细胞抵抗次黄嘌呤 - 黄嘌呤氧化酶诱导的损伤有保护作用。
研究结果表明，姜黄素确实对人角质形成细胞和成纤维细胞中的过氧化氢损伤具有强大的抑制作用。
