Tsai C G, Jones G A
Can J Microbiol. 1975 Jun;21(6):794-801. doi: 10.1139/m75-117.
Eight strains of rumen bacteria capable of degrading phloroglucinol (1,3,5-trihydroxybenzene) under anaerobic conditions were isolated from enrichment cultures of the bovine rumen microflora established in a prereduced medium containing 0.02 M phloroglucinol. Five of the strains were facultatively anaerobic Gram-positive streptococci which were identified as Streptococcus bovis. Three strains of obligately anaerobic Gram-positive cocci were assigned to the genus Coprococcus. Anaerobic cultures of the Streptococcus bovis strains in a 40% rumen fluid medium initially containing 0.02 M phloroglucinol degraded 50-80% of the substrate within 2 days, whereas cultures of the Coprococcus strains degraded more than 80% of the substrate under the same conditions. The Streptococcus bovis strains were incapable of degrading phloroglucinol in brain heart infusion or in the medium of de Man, Rogosa, and Sharpe (MRS broth) incubated aerobically.
从在含有0.02M间苯三酚的预还原培养基中建立的牛瘤胃微生物区系富集培养物中，分离出8株能够在厌氧条件下降解间苯三酚（1,3,5 - 三羟基苯）的瘤胃细菌。其中5株为兼性厌氧革兰氏阳性链球菌，鉴定为牛链球菌。3株专性厌氧革兰氏阳性球菌归为粪球菌属。在最初含有0.02M间苯三酚的40%瘤胃液培养基中，牛链球菌菌株的厌氧培养物在2天内降解了50 - 80%的底物，而在相同条件下，粪球菌菌株的培养物降解了超过80%的底物。牛链球菌菌株在需氧培养的脑心浸液或德氏、罗氏和夏普培养基（MRS肉汤）中不能降解间苯三酚。
