Thompson L A, Gates D M, Ingledew W M, Jones G A
Appl Environ Microbiol. 1976 Jan;31(1):21-4. doi: 10.1128/aem.31.1.21-24.1976.
The Hungate anaerobic technique was used with a standard procedure for bacterial mutagenesis employing N-methyl-N-nitro-N'-nitrosoguanidine to obtain mutants of an obligate anaerobe. Three mutant strains were derived from a Coprococcus sp., strain Pe15, a rumen anaerobe capable of growing on phloroglucinol. The mutants did not grow on phloroglucinol but did degrade the compound in anaerobic washed-cell suspensions, producing the same end products in approximately the same proportions as the wild type. It was concluded that the mutants were blocked in a unique step or steps necessary for carbon skeleton or energy synthesis from phloroglucinol and not in formation of an enzyme involved in the pathway of phloroglucinol degradation.
采用亨盖特厌氧技术和使用N-甲基-N-硝基-N'-亚硝基胍进行细菌诱变的标准程序，以获得专性厌氧菌的突变体。三个突变菌株源自粪球菌属的Pe15菌株，这是一种能够在间苯三酚上生长的瘤胃厌氧菌。这些突变体不能在间苯三酚上生长，但在厌氧洗涤细胞悬液中确实能降解该化合物，产生与野生型大致相同比例的相同终产物。得出的结论是，这些突变体在从间苯三酚合成碳骨架或能量所需的一个或多个独特步骤中受阻，而不是在间苯三酚降解途径中涉及的酶的形成过程中受阻。
