Nelson K E, Pell A N, Schofield P, Zinder S
Department of Animal Science, Cornell University, Ithaca, New York 14853, USA.
Appl Environ Microbiol. 1995 Sep;61(9):3293-8. doi: 10.1128/aem.61.9.3293-3298.1995.
An anaerobic diplococcoid bacterium able to degrade hydrolyzable tannins was isolated from the ruminal fluid of a goat fed desmodium (Desmodium ovalifolium), a tropical legume which contains levels as high as 17% condensed tannins. This strain grew under anaerobic conditions in the presence of up to 30 g of tannic acid per liter and tolerated a range of phenolic monomers, including gallic, ferulic, and p-coumaric acids. The predominant fermentation product from tannic acid breakdown was pyrogallol, as detected by high-performance liquid chromatography and mass spectrometry. Tannic acid degradation was dependent on the presence of a sugar such as glucose, fructose, arabinose, sucrose, galactose, cellobiose, or soluble starch as an added carbon and energy source. The strain also demonstrated resistance to condensed tannins up to a level of 4 g/liter.
从一只以热带豆科植物鸡眼草（Desmodium ovalifolium）为食的山羊瘤胃液中分离出一种能够降解可水解单宁的厌氧双球菌。鸡眼草含有高达17%的缩合单宁。该菌株在厌氧条件下生长，每升培养基中可添加高达30克单宁酸，并且能耐受多种酚类单体，包括没食子酸、阿魏酸和对香豆酸。通过高效液相色谱和质谱检测发现，单宁酸分解的主要发酵产物是连苯三酚。单宁酸的降解依赖于添加糖（如葡萄糖、果糖、阿拉伯糖、蔗糖、半乳糖、纤维二糖或可溶性淀粉）作为碳源和能源。该菌株对高达4克/升的缩合单宁也表现出抗性。
