Taulavuori E, Hellström E K, Taulavuori K, Laine K
Department of Biology, University of Oulu, PO Box 3000, FIN-90014 Oulu, Finland.
J Exp Bot. 2001 Dec;52(365):2375-80. doi: 10.1093/jexbot/52.365.2375.
Malondialdehyde (MDA) concentration is a widely used method to analyse lipid peroxidation in biological material. In plant tissues, however, certain compounds (anthocyanins, carbohydrates) may interfere with measurements which may lead to an overestimation of the MDA levels. Two methods were compared for analysing lipid peroxidation, either uncorrected or corrected for interfering compounds. The comparison was performed in three separate experiments with respect to cold treatments (snow removal in winter, reacclimation in summer and cold acclimation in autumn) in bilberry (Vaccinium myrtillus L.). During winter and autumn the methods seem to measure different compounds, but during active growth in the summer the difference between the methods was less. This is obviously due to carbohydrates which act as cryoprotectants and increase in concentration during cold acclimation as well as due to the anthocyanins. It is thus suggested that the validity of the uncorrected method to measure MDA and thereby lipid peroxidation is best in plant tissue which is in an active growth state.
丙二醛（MDA）浓度是分析生物材料中脂质过氧化作用的一种广泛使用的方法。然而，在植物组织中，某些化合物（花青素、碳水化合物）可能会干扰测量，这可能导致对MDA水平的高估。比较了两种分析脂质过氧化作用的方法，一种是未校正干扰化合物的方法，另一种是校正干扰化合物的方法。在蓝莓（越桔）的三个单独实验中，针对冷处理（冬季除雪、夏季再驯化和秋季冷驯化）进行了比较。在冬季和秋季，这两种方法似乎测量的是不同的化合物，但在夏季活跃生长期间，两种方法之间的差异较小。这显然是由于碳水化合物作为抗冻剂，在冷驯化过程中浓度增加，以及由于花青素。因此，建议在处于活跃生长状态的植物组织中，未校正方法测量MDA从而测量脂质过氧化作用的有效性最佳。
