Bao Li, Yao Xin-Sheng, Yau Chin-Chin, Tsi Daniel, Chia Chew-Sern, Nagai Hajime, Kurihara Hiroshi
College of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenhe District, Shenyang 110016, China.
J Agric Food Chem. 2008 Sep 10;56(17):7803-7. doi: 10.1021/jf800728m. Epub 2008 Aug 9.
Our experiments showed that 18 h restraint stress could induce serious liver damage, with an increase in plasma alanine aminotransferase (ALT) level (107.68 +/- 3.19 U/L vs 18.08 +/- 1.46 U/L). Meanwhile, we observed increased malondialdehyde (MDA) levels and lowered oxygen radical absorbance capacity (ORAC) values in plasma and liver of restraint mice compared with starved mice. Bilberry extract (containing 42.04% anthocyanins) was oral administrated to mice at 50, 100, and 200 mg/(kg x day) for five days, which remarkably decreased plasma ALT level to 17.23 +/- 2.49 U/L at the dose of 200 mg/(kg x day) and thus alleviated stress-induced liver damage. In addition, bilberry extracts increased glutathione (GSH) and vitamin C levels and significantly decreased MDA and nitric oxide (NO) levels in the liver tissues. These results suggest that bilberry extract plays an important role in protecting against restraint stress-induced liver damage by both scavenging free radicals activity and lipid peroxidation inhibitory effect. This study showed the beneficial health effects of bilberry extract through its antioxidative action.
我们的实验表明，18小时的束缚应激可导致严重的肝损伤，血浆丙氨酸转氨酶（ALT）水平升高（107.68±3.19 U/L对18.08±1.46 U/L）。同时，我们观察到与饥饿小鼠相比，束缚小鼠血浆和肝脏中的丙二醛（MDA）水平升高，氧自由基吸收能力（ORAC）值降低。用含有42.04%花青素的越橘提取物以50、100和200 mg/(kg·天)的剂量给小鼠口服五天，在200 mg/(kg·天)的剂量下，血浆ALT水平显著降至17.23±2.49 U/L，从而减轻了应激诱导的肝损伤。此外，越橘提取物提高了肝脏组织中谷胱甘肽（GSH）和维生素C的水平，并显著降低了MDA和一氧化氮（NO）的水平。这些结果表明，越橘提取物通过清除自由基活性和抑制脂质过氧化作用，在预防束缚应激诱导的肝损伤中发挥重要作用。本研究显示了越橘提取物通过其抗氧化作用对健康有益。
