Ertingshausen G, Shapiro S I, Green G, Zborowski G
Clin Chem. 1975 Aug;21(9):1305-13.
We report the adaptation of four radioassays to the prototype of an automated radioimmunoassay system ("Centria," Union Carbide). The system consists of three integrated modules: (a) an automated pipettor, which dispenses samples and reagents; (b) the key module, an incubator/separator, in which centrifugal force is used to initiate and terminate multiple radioassay incubations and separations simultaneously; and (c) a gamma-counter/computer, which counts three tubes simultaneously and converts counts into concentration units. Radioimmunoassays for thyroxine, triiodothyronine, and digoxin were developed with use of well-characterized antibodies and of prepackaged Sephadex-containing columns to separate bound and free radioactive ligand. A triiodothyronine-uptake test in which the same kind of columns were used was also adapted to the instrument. Results for clinical samples compared favorably with those obtained by manual procedures. We report data on correlation between different methods and preliminary data on precision of the prototype system.
我们报告了将四种放射免疫测定法适配于一种自动放射免疫测定系统（“Centria”，联合碳化物公司）的原型。该系统由三个集成模块组成：（a）一个自动移液器，用于分配样品和试剂；（b）关键模块，一个孵育器/分离器，利用离心力同时启动和终止多个放射免疫测定的孵育和分离过程；（c）一个γ计数器/计算机，可同时对三个试管进行计数并将计数转换为浓度单位。利用特性明确的抗体和预包装的含葡聚糖凝胶柱来分离结合型和游离型放射性配体，开发了甲状腺素、三碘甲状腺原氨酸和地高辛的放射免疫测定法。一种使用相同类型柱的三碘甲状腺原氨酸摄取试验也适配于该仪器。临床样本的检测结果与手工操作获得的结果相比具有优势。我们报告了不同方法之间的相关性数据以及该原型系统精密度的初步数据。
