Chen I W, Maxon H R, Heminger L A, Ellis K S, Volle C P
J Nucl Med. 1980 Dec;21(12):1162-8.
Two fully automated radioimmunoassay systems with batch and sequential modes of analysis were used to assay serum thyroxine, triiodothyronine, and digoxin. The results obtained were compared with those obtained by manual methods. The batch system uses antibody coated tubes while the sequential system uses immobilized antibody chambers for the separation of bound from free ligands. In accuracy, both systems compared favorable with the established manual methods, but the sequential system showed better precision than the batch system. There was a statistically significant carryover of thyroxine in the sequential system when there were at least six-fold differences in the concentrations of thyroxine in adjacent samples, but the carryover was not significant in the batch system. Compared with the batch system, the sequential system has a shorter throughtime for individual samples (time from aspiration of the sample to the printout of results) but a longer interval for final overall printout of assay results (lower throughput).
使用两个具有批量和连续分析模式的全自动放射免疫分析系统来测定血清甲状腺素、三碘甲状腺原氨酸和地高辛。将获得的结果与通过手工方法获得的结果进行比较。批量系统使用抗体包被管，而连续系统使用固定化抗体室来分离结合态和游离配体。在准确性方面，两个系统与既定的手工方法相比都表现良好，但连续系统比批量系统显示出更好的精密度。当相邻样品中甲状腺素浓度存在至少六倍差异时，连续系统中甲状腺素有统计学上显著的残留，但在批量系统中残留不显著。与批量系统相比，连续系统单个样品的通过时间较短（从吸取样品到打印结果的时间），但分析结果最终总体打印的间隔较长（通量较低）。
